In planta pathobiota of grapevines

Endophytic (in leaves, flowers and berries) and soil mycobiota of diseased and asymptomatic grapevines are compared by different methods. Selective fluorescent staining and microscopy is used for the localization of fungi in grapevine tissues, while traditional isolation-based methods and mass sequencing of fungal-specific amplicons are used to characterize the pathobiota in details.

Culture-dependent method of the endophytic microbiota examination